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Evaluation the Effect of Obesity Induced by High-Fat Diet on Lipid Profile, Oxidative Stress Indices and Cell Apoptosis in Wistar Rats and Ovarian Cancer Cell Line
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Atefe Sepehrian   , Reza Motaghipur , Ahmad Ghasemi *1 |
| 1- gonabad university of medical sciences , ghasemi.a51@gmail.com |
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Abstract: (335 Views) |
The main purpose of the current research is to determine the effects of obesity induced by high fat diet (HFD) on lipid profile and oxidative stress in plasma and ovarian samples of Wistar rats and to investigate its anti-apoptotic effects in ovarian cancer cells. In the animal study, female Wistar rats were divided into two groups fed with HFD and fed with standard control diet (SCD). After 14 weeks, mice in two groups were compared in terms of weight, biochemical factors, oxidative stress factors and ovarian tissue section. Also, in the cell culture model, the effect of serum samples taken from two groups on inhibition of cell apoptosis in SKOV3 cells has been investigated. Significant changes were observed in the ovarian tissue of two groups. HFD feeding increased mice weight, (P ˂0.01) glucose (P ˂0.05) and triglyceride (P ˂0.01) levels compared to SCD. Also, high levels of MDA (131±6.1 nmol/gr/tissue compared to 72.4±5.4) and decreased activity of SOD (525.3±23U/min/gr tissue compared to 189±23) and CAT enzymes (7.2±16 U/min/gr tissue compared to 16.2±2/3) were observed in the plasma and ovaries of rats fed with HFD compared to SCD. Cell treatment with serum samples from HFD-fed mice significantly inhibited cell apoptosis. Overall, our findings indicated that HFD-induced obesity may play an important role in ovarian cancer progression through changes in lipid profile and oxidative stress factors.
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| Keywords: Obesity, Lipid profile, Oxidative stress, Cell apoptosis, Ovarian cancer |
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Full-Text [PDF 704 kb]
(72 Downloads)
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Type of Study: Original |
Subject:
Special
Received: 2024/08/11 | Accepted: 2024/11/26 | Published: 2024/11/26
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Ethics code: IR.IUA.MSHD. REC.1398.229
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