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Association between MMP-2 rs243866 polymorphism with susceptibility to gastritis and Helicobacter pylori infection
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Marjan Soltani1    , Rasoul Zahmatkesh *2 , Eisa Jorjani1 |
1- Department of Biology, Faculty of Basic Science, Gonbad Kavous University, Iran.
2- Department of Cellular and molecular biology, To.C., Islamic Azad University, Tonekabon, Iran. , zahmatkesh.rasoul@gmail.com |
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Abstract: (72 Views) |
Background: Helicobacter pylori infection is one of the most important risk factors for gastritis. Gastric mucosa damage due to inflammation is associated with destruction of the extracellular matrix. Gastric epithelial cells infected with Helicobacter pylori stimulate the production of MMP-2. The occurrence of polymorphisms in the MMP-2 promoter affects its expression levels. The aim of this study was to investigate the association between rs2285053 and rs243866 polymorphisms in the MMP-2 promoter with gastritis and Helicobacter pylori infection.
Methods: This case-control study was conducted on 103 patients with gastritis with Helicobacter pylori infection and 116 healthy individuals as a control group. After genomic DNA extraction from the tissue, the genotypic frequency was determined by PCR-RFLP method. Statistical analysis was performed using SPSS software (version 26) with a significance level less than 0.05.
Results: The frequency of the AA genotype in patients was significantly higher than in the control group. a statistically significant difference was observed in the frequency of the AA genotype (χ2=12.16, 95%CI=1.48-71, OR=2.64, P=0.0002) and the allele A (χ2=17.58, 95%CI=1.27-34.1, OR=1.74, P=0.0004) at the rs243866 between the two studied groups.
Conclusion: The results showed that the AA genotype increases the likelihood of developing gastritis and Helicobacter pylori infection. However, further studies with a larger sample size and examining other functional polymorphisms of the MMP-2 gene seem necessary.
Keywords: Gastritis, Helicobacter pylori, Polymorphism, MMP-2.
Acknowledgements: This study was derived from the Master’s thesis of Ms. Marjan Soltani (Research Code: 197878), Islamic Azad University, Gonbad-e Kavous Branch, Iran. We would like to thank the staff of the Molecular Genetics Research Laboratory, Islamic Azad University, Tonekabon Branch, for their assistance. We also appreciate all physicians and staff of the endoscopy centers for providing the study samples. Finally, we sincerely thank all patients and healthy individuals who participated in and contributed to this research.
Data Availability Statement: The data and materials supporting the findings of this study are available from the corresponding author upon reasonable request.
Conflict of Interest: The authors declare that they have no conflicts of interest related to the present study.
Consent for Publication: Informed consent for participation in the study and for publication of the manuscript was obtained from all participants.
Ethics Approval and Consent to Participate: This study was approved by the Ethics Committee of the School of Medical Sciences, Islamic Azad University, Tonekabon Branch, Iran (Ethics Code: IR.IAU.TON.REC.1402.007). The study was conducted in accordance with the ethical principles of the Declaration of Helsinki. Before enrollment, the aims and procedures of the study were fully explained to all participants, and written informed consent was obtained from each participant. Confidentiality of participants’ information and protection of their privacy were maintained throughout all stages of the research.
Funding: This research received no specific grant from any funding agency in the public, commercial, or not-for-profit sectors.
Authors’ Contributions: Study design and methodology: Rasoul Zahmatkesh Roodsari. Sample collection and laboratory experiments: Marjan Soltani.Research supervision: Rasoul Zahmatkesh Roodsari and Isa Gerjani. Data analysis: Rasoul Zahmatkesh Roodsari and Marjan Soltani. All authors contributed to the drafting and initial editing of the manuscript, reviewed and approved the final version, and agree to be accountable for all aspects of the work. |
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Type of Study: Original |
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Research code: 197878 Ethics code: IR.IAU.TON.REC.1402.007
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Open Access Statement: This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (CC BY 4.0), which permits unrestricted use, distribution, and reproduction in any medium or format, provided that the original work is properly cited.
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