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:: Volume 1, Issue 2 (12-2023) ::
Health Res Develop 2023, 1(2): 1-8 Back to browse issues page
Association between IL-4 Gene VNTR Polymorphism and Type 2 Diabetes
Ramin Nasrollahzadeh1 , Mohammad Amin Momeni Moghaddam2 , Davood Salarbashi2 , Milad Khorasani *3
1- Medical Student, Faculty of Medicine, Gonabad University of Medical Sciences, Gonabad, Iran, Medical Student, Faculty of Medicine, Gonabad University of Medical Sciences, Gonabad, Iran
2- Department of Biochemistry, Nutrition and Food Industries, Faculty of Medicine, Gonabad University of Medical Sciences, Gonabad, Iran, Department of Biochemistry, Nutrition and Food Industries, Faculty of Medicine, Gonabad University of Medical Sciences, Gonabad, Iran
3- Department of Biochemistry and Nutrition, Neyshabur University of Medical Sciences, Neyshabur, Iran , Miladkh24@yahoo.com
Abstract:   (784 Views)
Abstract:
Background and Objective: Diabetes mellitus is a prevalent disease affecting a large population worldwide. Inflammation plays a crucial role in the development and progression of this disease. Interleukin-4 (IL-4) is an anti-inflammatory cytokine that can reduce inflammation. The aim of this study was to investigate the association between IL-4 gene VNTR polymorphism and type 2 diabetes in Gonabad city, Iran.
Methods:
This study was a case-control study. One hundred individuals who referred to Alameh Behlol Hospital Gonabad city of Iran were included in two groups: case (patients with type 2 diabetes) and control (healthy individuals). The participants were randomly selected and entered the study after obtaining informed consent and meeting the inclusion criteria. Two milliliters of blood were collected from each participant using EDTA-containing tubes, and DNA was extracted using the salting-out method. To investigate the VNTR polymorphism, the target fragment was first amplified using appropriate primers and then identified using electrophoresis.
Results:
The mean age of the study participants was 61.98 ± 7.30 years, and half of them were male. The results of the VNTR polymorphism analysis demonstrated no significant association between IL-4 gene VNTR polymorphism and type 2 diabetes (p > 0.05).
Conclusion:
In our study population, we found no significant association between IL-4 gene VNTR polymorphism and type 2 diabetes.

 
 

 
Keywords: Polymorphism, Interleukin-4, Type 2 Diabetes
Full-Text [PDF 954 kb]   (260 Downloads)    
Type of Study: Original | Subject: Special
Received: 2023/11/13 | Accepted: 2023/12/13 | Published: 2023/12/31
References
1. Tomic D, Shaw JE, Magliano DJ. The burden and risks of emerging complications of diabetes mellitus. Nature Reviews Endocrinology. 2022;18(9):525-539. [DOI:10.1038/s41574-022-00690-7]
2. Carracher AM, Marathe PH, Close KL. International diabetes federation 2017. Wiley Online Library; 2018. [DOI:10.1111/1753-0407.12644]
3. Khan RMM, Chua ZJY, Tan JC, Yang Y, Liao Z, Zhao Y. From pre-diabetes to diabetes: diagnosis, treatments and translational research. Medicina. 2019;55(9):546. [DOI:10.3390/medicina55090546]
4. Esteghamati A, Etemad K, Koohpayehzadeh J, Abbasi M, Meysamie A, Noshad S, et al. Trends in the prevalence of diabetes and impaired fasting glucose in association with obesity in Iran: 2005-2011. Diabetes research and clinical practice. 2014;103(2):319-327. [DOI:10.1016/j.diabres.2013.12.034]
5. Buraczynska M, Buraczynska K, Zukowski P, Ksiazek A. Interleukin-4 Gene Intron 3 VNTR Polymorphism in Type 2 Diabetes Patients with Peripheral Neuropathy. Immunol Invest. 2018 Feb;47(2):146-153. eng. Epub 20171128. doi:10.1080/08820139.2017.1407334. Cited in: Pubmed; PMID 29182400. [DOI:10.1080/08820139.2017.1407334]
6. Mirzaei M, Rahmaninan M, Mirzaei M, Nadjarzadeh A, Dehghani Tafti AA. Epidemiology of diabetes mellitus, pre-diabetes, undiagnosed and uncontrolled diabetes in Central Iran: results from Yazd health study. BMC public health. 2020;20:1-9. [DOI:10.1186/s12889-020-8267-y]
7. Chatterjee S, Khunti K, Davies MJ. Type 2 diabetes. The lancet. 2017;389(10085):2239-2251. [DOI:10.1016/S0140-6736(17)30058-2]
8. Pillon NJ, Loos RJ, Marshall SM, Zierath JR. Metabolic consequences of obesity and type 2 diabetes: Balancing genes and environment for personalized care. Cell. 2021;184(6):1530-1544. [DOI:10.1016/j.cell.2021.02.012]
9. Zhang D, Tang Z, Huang H, Zhou G, Cui C, Weng Y, et al. Metabolic regulation of gene expression by histone lactylation. Nature. 2019;574(7779):575-580. [DOI:10.1038/s41586-019-1678-1]
10. De Luca C, Olefsky JM. Inflammation and insulin resistance. FEBS letters. 2008;582(1):97-105. [DOI:10.1016/j.febslet.2007.11.057]
11. Oguntibeju OO. Type 2 diabetes mellitus, oxidative stress and inflammation: examining the links. International journal of physiology, pathophysiology and pharmacology. 2019;11(3):45.
12. Khdair SI, Al-Naimat OS, Jarrar W, Al-Qerem W, Khudeir FA. The Influence of TNF-α, IL-6, TGF-β1, IFN-γ, IL-10 Polymorphisms on Predisposition to Diabetes Mellitus among Jordanian Patients. Endocrine, Metabolic & Immune Disorders-Drug Targets (Formerly Current Drug Targets-Immune, Endocrine & Metabolic Disorders). 2023;23(5):681-691. [DOI:10.2174/1871530322666220827143530]
13. Creusot RJ, Chang P, Healey DG, Tcherepanova IY, Nicolette CA, Fathman CG. A Short Pulse of IL-4 Delivered by DCs Electroporated With Modified mRNA Can Both Prevent and Treat Autoimmune Diabetes in NOD Mice. Molecular Therapy. 2010 2010/12/01/;18(12):2112-2120. doi: https://doi.org/10.1038/mt.2010.146 [DOI:10.1038/mt.2010.146.]
14. Michurina S, Stafeev I, Beloglazova I, Zubkova E, Mamontova E, Kopylov A, et al. Regulation of glucose transport in adipocytes by interleukin-4. Journal of Interferon & Cytokine Research. 2022;42(3):127-136. [DOI:10.1089/jir.2021.0213]
15. Kannourakis G, Abbas A. The role of cytokines in the pathogenesis of Langerhans cell histiocytosis. The British journal of cancer Supplement. 1994;23:S37.
16. Salimi S, Mohammadoo-Khorasani M, Yaghmaei M, Mokhtari M, Moossavi M. Possible association of IL-4 VNTR polymorphism with susceptibility to preeclampsia. BioMed Research International. 2014;2014. [DOI:10.1155/2014/497031]
17. Phu TA, Ng M, Vu NK, Bouchareychas L, Raffai RL. IL-4 polarized human macrophage exosomes control cardiometabolic inflammation and diabetes in obesity. Molecular Therapy. 2022 2022/06/01/;30(6):2274-2297. doi: https://doi.org/10.1016/j.ymthe.2022.03.008 [DOI:10.1016/j.ymthe.2022.03.008.]
18. Ibrahimi M, Moossavi M, Mojarad EN, Musavi M, Mohammadoo-Khorasani M, Shahsavari Z. Positive correlation between interleukin-1 receptor antagonist gene 86bp VNTR polymorphism and colorectal cancer susceptibility: a case-control study. Immunologic research. 2019;67:151-156. [DOI:10.1007/s12026-018-9034-3]
19. Ali R, Saber L, Al-Harbi A. A novel association between IL1-Ra (receptor antagonist) gene polymorphism and T1DM in Al-Madina Al-Mounawra. European Review for Medical and Pharmacological Sciences. 2015;19(19):3701-3708.
20. Mohammadoo-Khorasani M, Salimi S, Tabatabai E, Sandoughi M, Zakeri Z. Association of interleukin-1 receptor antagonist gene 86bp VNTR polymorphism with systemic lupus erythematosus in south east of Iran. Zahedan Journal of Research in Medical Sciences. 2013;16(12).
21. Badr E, Assar M, Elshayeb EI, Fath El-Bab S, El-Kousy S. A preliminary study of the relation between IL-4 and hypertension in type II diabetes mellitus. Molecular biology reports. 2018;45(6):1967-1972. [DOI:10.1007/s11033-018-4349-7]
22. Tripathi A, Shukla S, Tripathi J, Saket R, Kol S, Mishra P, et al. Association of genetic polymorphism of inflammatory genes (IL-1β and IL-4) with diabetes type 2. Enliven: J Genet Mol Cell Biol. 2015;1(1):004. [DOI:10.18650/2379-5700.21001]
23. Daneshmandi S, Pourfathollah AA, Kazemi Arababadi M, Hasan Shahi GH, Rezaeian M, Asyabanha Rezaee M. Evaluation of relation between IL-4 and IFN- γ polymorphisms and type 2 diabetes [Research(Original)]. Journal of Mazandaran University of Medical Sciences. 2008;18(66):35-41. eng.
24. Hassan MM. The significance of interleukin 4 (IL-4)(590-C/T) gene polymorphism in Iraqi patients with type 2 diabetes mellitus: A case-control study. Res J Pharm Technol. 2019;12(11):5133-5137. [DOI:10.5958/0974-360X.2019.00889.8]
25. Ho K-T, Shiau M-Y, Chang Y-H, Chen C-M, Yang S-C, Huang C-N. Association of interleukin-4 promoter polymorphisms in Taiwanese patients with type 2 diabetes mellitus. Metabolism. 2010 2010/12/01/;59(12):1717-1722. doi: https://doi.org/10.1016/j.metabol.2010.04.010 [DOI:10.1016/j.metabol.2010.04.010.]
26. Ali R, El‐Said A, El‐Baz H, Settin A. Ethnic variation of IL‐4 intron 3 VNTR gene polymorphism; its association with type 2 diabetes mellitus and its complication (neuropathy) in Egyptian subjects. American Journal of Medical Genetics Part B: Neuropsychiatric Genetics. 2018;177(7):635-640. [DOI:10.1002/ajmg.b.32647]
27. Kazemi AM. Interleukin-4 gene polymorphisms in type 2 diabetic patients with nephropathy. Iranian Journal of Kidney Diseases. 2010;4(4):302-306.
28. Basol N, Inanir A, Yigit S, Karakus N, Kaya SU. High Association of IL-4 Gene Intron 3 VNTR Polymorphism with Diabetic Peripheral Neuropathy. Journal of Molecular Neuroscience. 2013 2013/10/01;51(2):437-441. doi:10.1007/s12031-013-0048-y. [DOI:10.1007/s12031-013-0048-y]
29. Neelofar K, Ahmad J, Ahmad A, Alam K. Study of IL4-590C/T and IL6-174G/C Gene Polymorphisms in Type 2 Diabetic Patients With Chronic Kidney Disease in North Indian Population. Journal of Cellular Biochemistry. 2017;118(7):1803-1809. doi: https://doi.org/10.1002/jcb.25853 [DOI:10.1002/jcb.25853.]
30. Arababadi MK, Nosratabadi R, Hassanshahi G, Yaghini N, Pooladvand V, Shamsizadeh A, et al. Nephropathic complication of type-2 diabetes is following pattern of autoimmune diseases? Diabetes research and clinical practice. 2010;87(1):33-37. [DOI:10.1016/j.diabres.2009.09.027]
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Nasrollahzadeh R, Momeni Moghaddam M A, Salarbashi D, Khorasani M. (2023). Association between IL-4 Gene VNTR Polymorphism and Type 2 Diabetes. Health Res Develop. 1(2), 1-8. doi:10.61186/jhrd.1.2.1
URL: http://jhrd.trjums.ac.ir/article-1-31-en.html
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Volume 1, Issue 2 (12-2023) Back to browse issues page
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